assays effectene transfection reagent qiagen (Thermo Fisher)

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Thermo Fisher assays effectene transfection reagent qiagen
Assays Effectene Transfection Reagent Qiagen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/effectene/1+AMINO+1+CYCLOPROPANECA+250MG+250MG/pm40911418-300-271-282
Average 93 stars, based on 1 article reviews

assays effectene transfection reagent qiagen - by Bioz Stars, 2026-11

93/100 stars

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Transfection:

Article Title: Spontaneous Neurotransmitter Release Shapes Dendritic Arbors via Long-Range Activation of NMDA Receptors
Article Snippet: .. After 3–6 DIV, neurons were transfected using Effectene (Invitrogen). ..

Article Title: The upstream conserved regions (UCRs) mediate homo- and hetero-oligomerization of type 4 cyclic nucleotide phosphodiesterases (PDE4s)
Article Snippet: .. For expression of recombinant protein, cells were transfected with recombinant vector DNA using Effectene (Life Technologies) or LipofectamineTM 2000 (FRET experiments; Life Technologies), or infected with adenoviruses at an MOI (multiplicity of infection) of 100. .. GST and GST-fusion proteins were expressed in BL21(DE3) Escherichia coli cells transformed with pGEX5x-3 vectors and were purified using glutathione–Sepharose (GE Healthcare).

Article Title: Polyploidy road to therapy-induced cellular senescence and escape.
Article Snippet: Histone H2B-RFP (H2B-RFP) in pENTR1A was obtained from Addgene (Cambridge, MA) and subcloned into pZeoSV2. .. Cells were transfected with pZeoSV2-H2B-RFP with Effectene (Invitrogen, Carlsbad, CA) following the manufacture’s instruction and stable expression cell line was obtained by Zeocin selection and single cell culture. .. Plasmids pCS2-p21 and p27 are kind gifts from John Grim (Fred Hutchinson Cancer Research Center, Seattle, WA 98109) and transfected with Effectene following the manufacturer’s protocol (Invitrogen, Carlsbad, CA).

Article Title: ARMC5 Variants and Risk of Hypertension in Blacks: MH‐GRID Study
Article Snippet: Human embryonic kidney 293 (HEK293) cells were grown in Dulbecco’s modified Eagle medium (DMEM) (11995, Gibco) enriched with 10% fetal bovine serum (FBS) (900, Gemini Bio-Products Inc), GlutaMAX (35050, Gibco), and Anti (15240, Gibco), whereas human adrenocarcinoma (H295R) cells were maintained in DMEM-F12 (11320, Gibco) containing 10% FBS (900, Gemini Bio-Products Inc), GlutaMAX (35050, Gibco), Anti (15240, Gibco), and Insulin-TransferrinSelenium (41400, Thermo Fisher Scientific). .. A total of 300 000 HEK293 or 400 000 H295R cells were seeded in a well of 6-well plate and were transfected the next day with 1 lg of empty, wild-type or mutant plasmid using Lipofectamine LTX (15338100, Thermo Fisher Scientific) or Effectene (301425, Qiagen), respectively. .. Where indicated, cells were treated with 10 ng of cycloheximide (C4859, Sigma-Aldrich) or DMSO (34869, Sigma-Aldrich) for the control for 1, 2, or 3 hours before collection.

Article Title: The RUNX1/IL-34/CSF-1R axis is an autocrinally regulated modulator of resistance to BRAF-V600E inhibition in melanoma.
Article Snippet: .. Melanoma cell lines stably expressing CSF1R promoter–luciferase constructs were generated via transfection with Effectene and selection with G418-Sulfate (Thermo Fisher Scientific). .. To produce the lentivirus, pGIPZ plasmids (Dharmacon) were cotransfected into HEK293T cells with the following helper plasmids: pRc/CMV, pHDM-Hgpm2, pHDM-tat1b, and pHDM-VSV-G, using Effectene transfection reagent (Qiagen).

Article Title: ARMC 5 Variants and Risk of Hypertension in Blacks: MH‐ GRID Study
Article Snippet: Human embryonic kidney 293 (HEK293) cells were grown in Dulbecco's modified Eagle medium (DMEM) (11995, Gibco) enriched with 10% fetal bovine serum (FBS) (900, Gemini Bio‐Products Inc), GlutaMAX (35050, Gibco), and Anti (15240, Gibco), whereas human adrenocarcinoma (H295R) cells were maintained in DMEM‐F12 (11320, Gibco) containing 10% FBS (900, Gemini Bio‐Products Inc), GlutaMAX (35050, Gibco), Anti (15240, Gibco), and Insulin‐Transferrin‐Selenium (41400, Thermo Fisher Scientific). .. A total of 300 000 HEK293 or 400 000 H295R cells were seeded in a well of 6‐well plate and were transfected the next day with 1 μg of empty, wild‐type or mutant plasmid using Lipofectamine LTX (15338100, Thermo Fisher Scientific) or Effectene (301425, Qiagen), respectively. .. Where indicated, cells were treated with 10 ng of cycloheximide (C4859, Sigma‐Aldrich) or DMSO (34869, Sigma‐Aldrich) for the control for 1, 2, or 3 hours before collection.

Expressing:

Recombinant:

Article Title: Epstein-Barr Virus gp350 Can Functionally Replace the Rhesus Lymphocryptovirus Major Membrane Glycoprotein and Does Not Restrict Infection of Rhesus Macaques
Article Snippet: The plates were washed 3 times with PBS–0.1% Tween 20 and blocked for 2 h with blocking solution (PBS, 0.1% Tween 20, 0.3% I-Block [Applied Biosystems]) at room temperature. .. Recombinant antibodies were produced by transiently transfecting 293 cells with pCIRN-H1/H2 and pEIG-L1/L2 using Effectene (Life Technologies) according to the manufacturer's protocol. ..

Plasmid Preparation:

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Knockdown:

Article Title: Gsk3β and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease
Article Snippet: .. Transient knockdown (KD) of Fbxo7 was performed by transfecting dsRNA targeting Fbxo7 into cells using Effectene (Invitrogen) as described previously [ ]. .. Transient knockdown (KD) of Fbxo7 was performed by transfecting dsRNA targeting Fbxo7 into cells using Effectene (Invitrogen) as described previously [ ].

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